In order to provide the basis of precise use of chemicals in disease control in culture of the whiteleg shrimp Penaeus vannamei in Ningde,the bacteria were isolated from the shrimp from 7 mari-culture farms,and identified by morphological characters,physiological and biochemical characteris-tics,and phylogenetic analysis of 16S rRNA gene.Then,the antibiotic sensitivity of the isolated bac-teria was tested.The results showed that 9 strains of pathogenic bacteria were blue-green in TCBS agar medium.Microscopically,Gram staining revealed Gram-negative non-sporulating bacteria with rod shape,arc shape and oval shape.Physiological and biochemical characteristics showed that the strain was positive in 8 experiments including oxidase reaction,glucose,lysine decarboxylase,or-nithine decarboxylase,mannitol,6％NaCl tryptone water,8％NaCl tryptone water,and motivity.The results demonstrated that the isolated bacteria was identified as Vibrio parahemolyticus.Antibi-otics susceptibility test showed that the 9 strains had high tolerance resistant to penicillin,neomycin and tetracycline,and were highly sensitive to 6 drugs such as enrofloxacin,furazolidone and vancomy-cin.
Penaeus vannameiVibrio parahemolyticusisolation and identificaitionantibiotics sus-ceptibility
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