宁德市南美白对虾副溶血弧菌的分离与药敏试验
Isolation,identification and antibiotic sensitivity of Vibrio parahaemolyticus in Penaeus vannamei from Ningde City
陈小强1
作者信息
- 1. 福建省闽东水产研究所,福建宁德 352100
- 折叠
摘要
为给宁德市南美白对虾(Penaeus vannmei)养殖病害提供精准用药依据,从7个海水养殖场南美白对虾体内分离病原菌,通过形态学观察、生理生化和16s rRNA鉴定,确定其分类地位,并进行药物敏感试验.结果表明,分离的9株病原菌在TCBS琼脂培养基中菌落均呈蓝-绿色,通过革兰氏染色镜检可见为革兰氏阴性菌,呈棒状、弧状、卵圆状,无芽孢.在氧化酶反应、葡萄糖、赖氨酸、鸟氨酸、甘露醇、6％盐胨水、8％盐胨水、动力等8项试验中,结果呈阳性,确定为副溶血弧菌.9株副溶血弧菌对青霉素、新霉素、四环素等3种药物耐受比例较高,对恩诺沙星、呋喃唑酮、万古霉素等6种药物均高度敏感.
Abstract
In order to provide the basis of precise use of chemicals in disease control in culture of the whiteleg shrimp Penaeus vannamei in Ningde,the bacteria were isolated from the shrimp from 7 mari-culture farms,and identified by morphological characters,physiological and biochemical characteris-tics,and phylogenetic analysis of 16S rRNA gene.Then,the antibiotic sensitivity of the isolated bac-teria was tested.The results showed that 9 strains of pathogenic bacteria were blue-green in TCBS agar medium.Microscopically,Gram staining revealed Gram-negative non-sporulating bacteria with rod shape,arc shape and oval shape.Physiological and biochemical characteristics showed that the strain was positive in 8 experiments including oxidase reaction,glucose,lysine decarboxylase,or-nithine decarboxylase,mannitol,6％NaCl tryptone water,8％NaCl tryptone water,and motivity.The results demonstrated that the isolated bacteria was identified as Vibrio parahemolyticus.Antibi-otics susceptibility test showed that the 9 strains had high tolerance resistant to penicillin,neomycin and tetracycline,and were highly sensitive to 6 drugs such as enrofloxacin,furazolidone and vancomy-cin.
关键词
南美白对虾(Penaeus/vanmmei)/副溶血弧菌(Vibrio/parahaemolyticus)/分离鉴定/药敏试验Key words
Penaeus vannamei/Vibrio parahemolyticus/isolation and identificaition/antibiotics sus-ceptibility引用本文复制引用
基金项目
福建省属公益类科研院所基本科研专项(2021R1036003)
宁德市现代海洋渔业发展专项资金项目(202102)
出版年
2024
NETL
NSTL
万方数据