Effects of pH,glucose and several cations on sperm motility of Benxi Brachymystax lenok Pallas
To provide a theoretical basis for the selection of the best fertilization activation medium in the artificial breeding of Benxi Manchurian trout(Brachymystax lenok Pallas),and also to provide a scientific basis for the preparation of sperm dilution and preservation solution of the fish,the effects of pH,glucose,metal ions such as Na+,K+,Ca2+,Mg2+and cold storage time on sperm motility of Benxi Manchurian trout were studied by plate slide method.The results showed that sperm should be stored at ultra-low temperatures,the activation solution of artificial insemination of Benxi Manchuri-an trout should be prepared with tap water,pH 8,and contain 0.5 g/L Na+,0.3 g/L Mg2+,0.3 g/L Ca21and 50 mmol/L glucose.It was concluded that the appropriate pH value and glucose,Na+,Mg2+,Ca2+can enhance sperm motility of Benxi Manchurian trout.K+can inhibit the sperm motility of Benxi Manchurian trout.Na+is the main factor promoting sperm motility of Benxi Manchurian trout.
万方数据
维普
