Isolation and identification of three probiotics and analysis of their bacteriostatic effect on aquatic pathogens and water purification
In order to screen probiotics that can be used in microecological preparations,three strains of microorganisms were successfully screened from the bottom mud of aquaculture ponds.Their biologi-cal characteristics,inhibition effect on pathogenic bacteria and purification ability on water environ-ment were deeply discussed.The isolated strains were identified by morphological observation and 16 S rRNA gene sequence comparison.The results showed that all the three strains were Bacillus.The growth performance of the three strains was measured,and the drug resistance and antibacterial effect were analyzed.The results showed that the three strains had the ability to inhibit Escherichia coli and Vibrio alginolyticus,and were sensitive to 9 antibiotics such as amoxicillin,erythromycin thiocya-nate,doxycycline hydrochloride and ampicillin.The content of nitrite(NO-2-N)and ammonia nitro-gen(NH-4-N)in water was determined.The results showed that the three strains had a certain effect on water purification.The three selected strains showed the biological characteristics required as aquatic feed additives and had the potential to improve water quality.
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维普
