In order to investigate the status quo of mandarin fishes in Hebei Province and provide better reference data for the protection of mandarin fishes germplasm resources in China,the mandarin fishes populations were identified at the molecular level and the correlation analysis of genetic diversity level was carried out with the support of mitochondrial COX1 and genome-wide SNP markers.The results showed that 40 homologous sequences of COX1 with a total length of 697 bp and two haplotype se-quences of SSH01 and SSH02 were obtained.The phylogenetic tree constructed by the maximum like-lihood method showed that the two haplotypes clustered first into one,and then clustered with Sini-perca scherzeri.In the phylogenetic tree of geographic population,two haplotypes were clustered to-gether with the reference to Changju S.scherzeri in Korea.The mean of observed heterozygosity(Ho)was 0.330,the mean of PIC was 0.269,and the mean of inbreeding coefficient was about 0.There was no significant difference in observed heterozygosity between SSH01 and SSH02(P＞0.05).The coancestry coefficient was 0 in 71.41%of the population,and there were more closely re-lated individuals.PCA analysis resnlts showed that the population can be divided into 3 genetic struc-tures,i.e.,Cluster0,Cluster1 and Cluster2;The Admixture showed that two ancestors were most likely present;The result of linkage disequilibrium analysis was that the Ne range of the population was 14.8～27.6 under 95%confidence interval.S.scherzeri in Hebei Province has two haplotypes with low population genetic diversity.S.scherzeri is closely related to Changju S.scherzeri in Korea.S.scherzeri in Hebei Province can be protected by artificial introduction and breeding and release.
维普
万方数据