In order to improve the survival rate of Epinephelus cyanopodus in transport,seedling grad-ing and marking in enhancement release,and determine the concentration of eugenol in anesthesia transport,the anesthetic effects of different mass concentrations of eugenol were determined on E.cyanopodus with body weight of(27.84±6.91)g.The effects of mass concentrations of eugenol at 5 mg/L and 9 mg/L on the physiological and biochemical indexes of liver were also determined.The re-sults showed that under the temperature of(29.5±0.3)℃,when the concentration of eugenol was 5 mg/L and 10 mg/L,the final anesthetic state of the fish was deep sedation and mild anesthesia,respec-tively,and the normal state was recovered within 4 minutes.In the anesthesia and resuscitation tests,there was no significant difference in liver biochemical indexes between the 5 mg/L group and the con-trol group,while SOD,CAT,ALT and AST in liver of the fish of the 9 mg/L group were significantly lower than those of the control group at 6 h after anesthesia,and the enzyme activities returned to nor-mal after 12 h of resuscitation in clear water,which proved that the effects were reversible.Therefore,eugenol with a mass concentration of 5 mg/L is optional for 12 h anesthetic transport.
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