Effects of dietary fatty acid calcium replacing fish oil and soybean oil on growth performance,immunity and antioxidant capacity in largemouth bass(Micropterus salmoides)
To investigate the effect of fatty acid calcium(CSFA)replacing fish oil and soybean oil on the immunity and antioxidant capacity of largemouth bass(Micropterus salmoides),300 healthy largemouth bass of uniform size,with body weight of(10.00±0.1)g/ind.had been domesticated com-pletely,and were randomly divided into five groups.Five kinds of isonitrogenous and isoenergetic diets were prepared,which were control(D1)group with equal levels of fish oil and soybean oil,CSFA re-placing fish oil(D2)group,CSFA replacing soybean oil(D3)group,all-CSFA(D4)group and all-soybean oil(D5)group.The experimental period was 56 d.The results showed that,the body weight growth rate of D1,D2 and D3 groups was significantly higher than that of the other groups(P<0.05).CSFA replacement of fish oil and soybean oil in diet had significant effects on the immunologi-cal and antioxidant indices of serum and liver of the fish(P<0.05).The hepatic acid phosphatase(ACP),alkaline phosphatase(AKP),superoxide dismutase(SOD),and catalase(CAT)activities in the liver of the fish followed a trend of increasing firstly and then decreasing.Among them,the activi-ties of hepatic immunoenzymes ACP,AKP and antioxidant enzymes CAT,SOD were the highest in group D3,and hepatic malondialdehyde(MDA)content was the lowest in group D3.Considering the growth,immunity and antioxidant indexes,it was concluded that the suitable group for largemouth bass artificial compound feed was group D3.