Effects of selenomethionine on glucose metabolism in serum and liver of juvenile largemouth bass(Micropterus salmoides)
The present study was conducted to investigate the effects of dietary supplementation with seleno-methionine(SeMet)on serum biochemical indices and hepatic glucose metabolism in juvenile largemouth bass(Micropterus salmoides).The base diet was supplemented with 1.6 mg/kg sodium selenite(SodSe),0.4,0.8,1.6 and 3.2 mg/kg SeMet to form five isonitrogenous and isoenergetic diets for juvenile largemouth bass for 56 days.The results showed that the glucose metabolism with dietary SeMet supplementation was superior to that of SodSe.Adequate SeMet significantly reduced serum and liver glucose levels compared with the con-trol group(P＜0.05).Moreover,dietary 0.8 mg/kg SeMet could promote glucose catabolism and utilization through activation of glucose transport(GLUT2)、glycolysis(GK,HK,PFK)and inhibition of gluconeogene-sis(PEPCK)compared to the control group(P＜0.05).In contrast,the expression of glycogen synthesis genes(gys,g3k3β)and hepatic glycogen content decreased(P＜0.05).In conclusion,these results suggested that the addition of 0.8 mg/kg SeMet can improve the glucose metabolism of largemouth bass and promote the utilisation of carbohydrates.
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