Separation identification and pathogenicity study of Pseudomonas putida from blood parrotfish
To help preventing disease caused by the bacterium Pseudomonas putida,a strain of bacteri-um was isolated from the kidney and liver of the blood parrotfish(Vieja synspila ♀× Amphilophus citrinellus ♂)in a farm in Tianjin,and the pathogen isolation and identification,artificial regression infection experiment and drug sensitivity test were conducted.The pathogenic bacteria were isolated from the lesion and named AC4.After artificial regression of infection,AC4 was proved to be patho-genic.The clinical symptoms were consistent with its natural symptoms,and the semi-lethal concen-tration was 3×108 CFU/mL.After biochemical identification of the isolated pathogenic bacteria,16S rRNA sequencing,the Gram staining of the bacteria showed Gram-negative rod-shaped bacteria,and the isolated bacteria belongs to the genus Pseudomonas.Susceptibility test showed that the strain was highly sensitive to aztreonam,ceftazidime,ceftriaxone,levofloxacin,cefepime,streptomycin,poly-myxin B,kanamycin,and amikacin.
Vieja synspila ♀ × Amphilophus citrinellus ♂Pseudomonas putidaseparation identifica-tiondrug sensitive test
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