Methods of improving inoculum quality of vitamin C two-step fermentation
The effects of L-cysteine or(and)calcium chloride on the growth and production of 2-keto-L-gulonic acid(2-KLG)in two-step culture medium of vitamin C were studied.The results showed that adding appropriate amount of L-cysteine and(or)calcium chloride to the two-step culture medium could promote the growth of Ketogulonigenium vulgare,increase the cell density,coordinate the mixed bacteria proportion and improve the quality of the mixed strains,the production rate of 2-keto-L-gulonic acid was increased and the fermentation period was shortened.The effect of adding 0.3 g/L cysteine and 0.6 g/L calcium chloride in the seed liquor culture medium was the most remarkable.The concentration of Ketogulonigenium vulgare in the seed liquor increased by 30.77％,and the fermentation period shortened by 8.33％,the formation rate of 2-keto-L-gulonic acid increased by 9.31％.
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