提高维生素C二步发酵种子质量的方法
Methods of improving inoculum quality of vitamin C two-step fermentation
胡静 1刘丽华 1朱娜 2苏宏儒 1王婧 1米帅东1
作者信息
- 1. 石药集团维生药业(石家庄)有限公司,河北石家庄 050000;石家庄市维生素C技术创新中心,河北石家庄 050000;河北省营养强化剂技术创新中心,河北石家庄 050000
- 2. 石家庄市食品药品检验中心,河北石家庄 050000
- 折叠
摘要
通过在维生素C二步种液培养基中添加半胱氨酸或(和)氯化钙的方法,研究了这2种物质对二步菌种生长和产2-酮基-L-古龙酸(2-KLG)的影响.结果表明,在二步种子培养基中加入适量的半胱氨酸和(或)氯化钙能够促进普通生酮基古龙酸菌生长,提高菌体密度,协调混菌比例,提高种子质量,并提高后续发酵2-酮基-L-古龙酸生成速率,缩短发酵周期.在种子培养基中同时加入0.3 g/L半胱氨酸和0.6g/L氯化钙培养效果最显著:种液中普通生酮基古龙酸菌浓度提高30.77％,发酵周期缩短8.33％,2-酮基-L-古龙酸生成速率提高9.31％.
Abstract
The effects of L-cysteine or(and)calcium chloride on the growth and production of 2-keto-L-gulonic acid(2-KLG)in two-step culture medium of vitamin C were studied.The results showed that adding appropriate amount of L-cysteine and(or)calcium chloride to the two-step culture medium could promote the growth of Ketogulonigenium vulgare,increase the cell density,coordinate the mixed bacteria proportion and improve the quality of the mixed strains,the production rate of 2-keto-L-gulonic acid was increased and the fermentation period was shortened.The effect of adding 0.3 g/L cysteine and 0.6 g/L calcium chloride in the seed liquor culture medium was the most remarkable.The concentration of Ketogulonigenium vulgare in the seed liquor increased by 30.77％,and the fermentation period shortened by 8.33％,the formation rate of 2-keto-L-gulonic acid increased by 9.31％.
关键词
二步发酵种子/半胱氨酸/氯化钙/普通生酮基古龙酸菌/菌体密度/2-酮基-L-古龙酸Key words
two-step mixed bacteria/L-cysteine/calcium chloride/ketoguloniogenium vulgare/cell density/2-keto-L-gulonic acid引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据