Determination of early frost resistance of different Zanthoxylum bungeanum varieties
In order to understand the early frost resistance of different Zanthoxylum bungeanum varieties,1-year old branches of'Jijiao No.1''Wudu Bayuejiao''Chaocang Shanjiao''Xinglong Qiujiao'were collected at the end of October before the tree was dormant.Indoor gradient cooling was carried out,and physiological indicators such as relative conductivity,malondialdehyde(MDA),osmoregulatory substances,and related antioxidant enzyme activities were measured.The results showed that:(1)After being subjected to low temperature stress,the relative conductivity of Zanthoxylum bungeanum branches showed an"S"curve.'Xinglong Qiujiao'had the lowest semi lethal temperature(-10.71℃),the strongest cold resistance,followed by'Chaocang shanjiao'(-7.79 ℃),and the highest semi lethal temperature was'Wudu Bayuejiao'(-4.167 ℃),indicating poor cold resistance;(2)After being subjected to low temperature stress,the MDA content in Zanthoxylum bungeanum branches showed a trend of first decreasing and then increasing;(3)The overall trend of osmotic regulatory substances in Zanthoxylum bungeanum branches under low temperature stress was a slow increase followed by a decrease;(4)After being subjected to low temperature stress,the SOD activity of Zanthoxylum bungeanum branches showed a trend of first increasing and then decreasing,then increasing and then decreasing;The overall trend of POD and CAT activity was an increase followed by a decrease;(5)The membership function analysis showed that the average membership ranking of the four Zanthoxylum bungeanum varieties was as follows:'Xinglong Qiujiao''Chaocang Shanjiao''Jijiao No.1''Wudu Bayuejiao'.Through comprehensive analysis,it can be concluded that the resis-tance to early frost of four Zanthoxylum bungeanum varieties,from strong to weak,was as follows:'Xinglong Qi-ujiao''Chaocang Shanjiao''Jijiao No.1''Wudu Bayuejiao'.
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