In order to make full use of the resources of jade bamboo,ultrasonic assisted hot water extraction method was used to extract polysaccharide from jade bamboo.Taking the extraction rate of Bamboos polysaccharides as the index,the three factors and levels that had the best effect on the extraction rate were selected through the single factor experiment on the extraction temperature,solid-liquid ratio,ultrasonic time and extraction time,and the orthogonal test was used to optimize the extraction process of bamboos polysaccharide.The results showed that the influence of single factor experiment on extraction rate was as follows:ultrasonic time>extraction temperature>solid-liquid ratio>extraction time.Orthogonal test was carried out to study the extraction process of Bambara polysaccharide.The optimum extraction temperature was 60℃,solid-liquid ratio was 1:30,ultrasonic time was 70min and extraction time was 90min.Under this condition,the extraction efficiency is the highest.By investigating the scavenging ability of polysaccharide on DPPH free radicals,it was found that polysaccharide had a good scavenging ability on DPPH free radicals,and the clearance rate reached a stable level at 50~60mg/mL.As a natural antioxidant,jade bamboo polysaccharide is expected to play a potential application value in food,medicine and cosmetics.
关键词
玉竹多糖/超声辅助/热水浸提法/提取率/工艺参数/抗氧化能力
Key words
Polysaccharides of Polygonatum Odoratum/Ultrasonic-assisted/Extraction Rate/Process Parameter/Antioxidant Capacity