Research on Optimization of Extraction Process and in Vitro Antioxidant Activity of Polygonatum Odoratum Polysaccharides
In order to make full use of the resources of jade bamboo,ultrasonic assisted hot water extraction method was used to extract polysaccharide from jade bamboo.Taking the extraction rate of Bamboos polysaccharides as the index,the three factors and levels that had the best effect on the extraction rate were selected through the single factor experiment on the extraction temperature,solid-liquid ratio,ultrasonic time and extraction time,and the orthogonal test was used to optimize the extraction process of bamboos polysaccharide.The results showed that the influence of single factor experiment on extraction rate was as follows:ultrasonic time＞extraction temperature＞solid-liquid ratio＞extraction time.Orthogonal test was carried out to study the extraction process of Bambara polysaccharide.The optimum extraction temperature was 60℃,solid-liquid ratio was 1:30,ultrasonic time was 70min and extraction time was 90min.Under this condition,the extraction efficiency is the highest.By investigating the scavenging ability of polysaccharide on DPPH free radicals,it was found that polysaccharide had a good scavenging ability on DPPH free radicals,and the clearance rate reached a stable level at 50～60mg/mL.As a natural antioxidant,jade bamboo polysaccharide is expected to play a potential application value in food,medicine and cosmetics.
Polysaccharides of Polygonatum OdoratumUltrasonic-assistedExtraction RateProcess ParameterAntioxidant Capacity
万方数据
维普
