辣椒辣素对大鼠炎性痛作用及脊髓TRPV1表达的影响
Effect of capsaicin on inflammatory pain and spinal TRPV1 expression in rats
吕传宝 1于莎莎 2李航兵 1董英伟1
作者信息
- 1. 哈尔滨医科大学附属第一医院麻醉科,黑龙江哈尔滨 150001
- 2. 哈尔滨医科大学附属第一医院疼痛科,黑龙江哈尔滨 150001
- 折叠
摘要
目的 观察辣椒辣素对炎性疼痛大鼠的镇痛作用,探讨其镇痛作用的可能机制.方法 将26只大鼠按照随机数字法分为4组,分别是对照组5例、模型组7例、低剂量组7例和高剂量组7例.模型组和给药组的大鼠在足跖中部皮下注射100µL弗氏完全佐剂(complete Freund's adjuvant,CFA),建立慢性炎性痛模型,动物造模后第2 d行硬膜外注射辣椒辣素,观察炎性疼痛大鼠机械痛阈,用丙酮喷雾法诱发疼痛并对疼痛程度进行评分.采用免疫组化SP法检测大鼠的背根神经节(dorsal root ganglion,DRG)组织中辣椒辣素受体(transient receptor potential vanilloid-1,TRPV1)蛋白的表达情况.结果 辣椒辣素提高了模型组大鼠机械痛阈和冷痛阈(P＜0.05),降低了大鼠的DRG组织中TRPV1蛋白的表达.结论 辣椒辣素对CFA诱导的大鼠慢性炎性痛有镇痛作用,其机制可能与下调TRPV1蛋白的表达有关.
Abstract
Objective To observe the analgesic effect of capsaicin on rats with inflammatory pain,and to explore its possible mechanism.Methods Twenty-six rats were divided into 4 groups according to random number method,namely control group(5 cases),model group(7 cases),low-dose group(7 cases)and high-dose group(7 cases).The rats in the model group and the administration group were subcutaneously injected with 100 μL complete Freund's ad-juvant(CFA)in the middle of the foot plantar to establish a chronic inflammatory pain model.The mechanical pain threshold of sexual pain rats was induced by acetone spray method and the pain degree was scored.The expression of transient receptor potential vanilloid-1(TRPV1)pro-tein in dorsal root ganglion(DRG)tissues of rats was detected by immunohistochemical SP method.Results Capsaicin increased the mechanical pain threshold and cold pain threshold of the rats in the model group(P＜0.05),and decreased the expression of TRPV1 protein in the DRG tissue of the rats.Conclusion Capsaicin has analgesic effect on the chronic inflam-matory pain induced by CFA in rats,and its mechanism may be related to the down-regulation of TRPV1 protein expression.
关键词
辣椒辣素/慢性炎性痛/痛阈/辣椒素受体Key words
capsaicin/chronic inflammatory pain/pain threshold/capsaicin receptor引用本文复制引用
基金项目
哈尔滨市科技攻关计划(2017AB9BS040)
出版年
2024
NETL
NSTL
万方数据