钛表面ZIF-L涂层的构建及细胞毒性检测
Construction and cytotoxicity test of ZIF-L coating on titanium
张善纯 1程祥玉 1宋蓉 1樊虹 1张润东 1任丽萍1
作者信息
- 1. 哈尔滨医科大学附属第一医院 口腔修复科,黑龙江哈尔滨 150001
- 折叠
摘要
目的 在钛(titanium,Ti)表面构建新型锌基金属有机骨架配合物(Ti@ZIF-L)涂层并进行生物学评价.方法 通过原位生长法在碱热处理后Ti试件表面构建锌基金属有机骨架材料(Ti@ZIF-L).利用扫描电镜观察材料表面形貌变化;接触角测量仪检测Ti和Ti@ZIF-L表面接触角;扭矩实验检测Ti@ZIF-L涂层的结合强度;利用细胞毒性实验评价Ti@ZIF-L的细胞相容性.结果 扫描电镜观察到Ti@ZIF-L表面形成多级、花状的纳米结构;接触角检测结果显示:Ti@ZIF-L接触角为38.11°,与未处理的Ti相比,接触角降低26.22°(P＜0.05);扭矩实验结果显示,在扭矩为9.48 N·m时,扫描电镜仍可见Ti表面生长的三维结构的ZIF-L,Ti与ZIF-L涂层间结合良好;细胞毒性实验显示,Ti与Ti@ZIF-L在不同浓度浸提液之间差异无统计学意义(P＞0.05).结论 Ti@ZIF-L涂层材料具有良好的稳定性和细胞相容性,有望应用于临床中对种植材料的表面改性.
Abstract
Objective To construct a novel zinc-based coating with a metal-organic framework complex(Ti@ZIF-L)on a titanium(Ti)surface and conduct the biological evaluation.Meth-ods Zinc-based metal-organic framework material was constructed on the surface of the Ti specimen after alkali heat treatment by in-situ growth method(Ti@ZIF-L).The surface mor-phology of the coating was observed by scanning electron microscopy(SEM),the surface con-tact angles of Ti and Ti@ZIF-L were measured by contact angle measuring instrument,and the bonding strength of Ti@ZIF-L was measured by torque test;The cytocompatibility of Ti@ZIF-L was evaluated by cytotoxicity assay.Results Multistage flower-like nanostructures were found on the surface of Ti@ZIF-L by SEM;The contact angle test results showed that the con-tact angle of Ti@ZIF-L was 38.11°,which was 26.22° lower than that of untreated Ti(P＜0.05);The results of the torque experiment showed that the three-dimensional structure of ZIF-L growing on the surface of Ti could still be seen by SEM when the torque was 9.48 N·m,in-dicating that the combination between Ti and ZIF-L coating was good;The cytotoxicity test showed that there was no statistical difference between Ti and Ti@ZIF-L at different concentra-tions(P＞0.05).Conclusion Ti@ZIF-L coating material has good stability and cell compati-bility and is expected to be used in clinical surface modification of implant materials.
关键词
钛/金属有机骨架/细胞毒性/种植体Key words
titanium/metal-organic skeleton/cytotoxicity/implant引用本文复制引用
基金项目
哈尔滨医科大学附属第一医院科研创新基金(2021M18)
黑龙江省卫生健康委科研项目(2020-118)
哈尔滨医科大学大学生创新创业训练计划(201910226454)
出版年
2024
NETL
NSTL
万方数据