Study on double network hydrogel loaded with baicalein for wound healing
Objective To develope a double network hydrogel loaded with baicalein,and to investigate its efficacy in enhancing wound healing.Methods PEGDA/CMCS@B hydrogel,composed of baicalein,polyethylene glycol diacrylate(PEGDA),and carboxymethyl chitosan(CMCS)was constructed by UV cross-linking and soaking method.The experimental materi-als were divided into 3 groups:PEGDA/CMCS group,PEGDA/CMCS@B0.5 group and PEG-DA/CMCS@B1.0 group,and their morphology was observed using scanning electron microsco-py(SEM).The antibacterial properties of the hydrogel were assessed against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)through inhibition zone experiments.Addi-tionally,cytocompatibility was evaluated by co-culturing L929 cells with three different hydro-gel formulations for 48 h,employing live/dead staining and CCK-8 assays.A full-thickness de-fect wound model was established on the back of mice,treated with different materials,and Im-age J software was used to analyze the wound repair situation in 15 days.Results SEM veri-fied that the PEGDA/CMCS@B double network hydrogel has a rich pore structure.The anti-bacterial experiment proved that PEGDA/CMCS@B1.0 hydrogel had better antibacterial effect(P＜0.001).Experiments on a rat full-thickness wound model confirmed that PEGDA/CMCS@B hydrogel can significantly promote wound healing.Conclusion The baicalein-load-ed double network hydrogel exhibits promising potential in the enhancement of wound healing processes.
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