负载黄芩素的双网络水凝胶用于创面愈合的研究
Study on double network hydrogel loaded with baicalein for wound healing
冯钰钦 1秦思 2李华润 2施思漫 3段忆雪 2许洁如 2蔡雪欣 3温诗瑜 2孙大炜 4温炬3
作者信息
- 1. 南方医科大学第二临床医学院 皮肤病与性病学,广东 广州 510515
- 2. 暨南大学附属广东省第二人民医院皮肤性病科,广东 广州 510317
- 3. 南方医科大学第二临床医学院 皮肤病与性病学,广东 广州 510515;暨南大学附属广东省第二人民医院皮肤性病科,广东 广州 510317
- 4. 暨南大学附属广东省第二人民医院创伤骨科,广东 广州 510317
- 折叠
摘要
目的 构建负载黄芩素的双网络水凝胶并对其促进创面愈合的机制进行研究.方法 将黄芩素(baicalein)、聚乙二醇二丙烯酸酯(polyethylene glycol diacrylate,PEGDA)、羧甲基壳聚糖(carboxym-ethyl chitosan,CMCS)等材料,通过紫外光交联及浸泡等方式,构建负载黄芩素的双网络水凝胶(PEGDA/CMCS@B),将实验分为PEGDA/CMCS、PEGDA/CMCS@B0.5 和PEGDA/CMCS@B1.0 3 组,运用扫描电子显微镜(scanning electron microscopy,SEM)观察其形态.选用大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)两种细菌,采用抑菌圈实验探究双网络水凝胶的抑菌性能.将L929 细胞与3 种不同水凝胶共培养48 h,运用活/死染色试验和CCK-8 检测,验证水凝胶的细胞相容性.在大鼠背部建立全皮层缺损创面模型,通过不同材料治疗,运用Image J软件分析 15 天创面修复情况.结果 SEM结果显示,PEGDA/CMCS@B双网络水凝胶具有丰富的孔隙结构.抑菌实验证明PEGDA/CMCS@B水凝胶具有更为出色的抑菌作用(P＜0.001).大鼠全层创面模型实验证实PEGDA/CMCS@B水凝胶可显著促进创面愈合.结论 负载黄芩素的双网络水凝胶可有效促进创面愈合.
Abstract
Objective To develope a double network hydrogel loaded with baicalein,and to investigate its efficacy in enhancing wound healing.Methods PEGDA/CMCS@B hydrogel,composed of baicalein,polyethylene glycol diacrylate(PEGDA),and carboxymethyl chitosan(CMCS)was constructed by UV cross-linking and soaking method.The experimental materi-als were divided into 3 groups:PEGDA/CMCS group,PEGDA/CMCS@B0.5 group and PEG-DA/CMCS@B1.0 group,and their morphology was observed using scanning electron microsco-py(SEM).The antibacterial properties of the hydrogel were assessed against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)through inhibition zone experiments.Addi-tionally,cytocompatibility was evaluated by co-culturing L929 cells with three different hydro-gel formulations for 48 h,employing live/dead staining and CCK-8 assays.A full-thickness de-fect wound model was established on the back of mice,treated with different materials,and Im-age J software was used to analyze the wound repair situation in 15 days.Results SEM veri-fied that the PEGDA/CMCS@B double network hydrogel has a rich pore structure.The anti-bacterial experiment proved that PEGDA/CMCS@B1.0 hydrogel had better antibacterial effect(P＜0.001).Experiments on a rat full-thickness wound model confirmed that PEGDA/CMCS@B hydrogel can significantly promote wound healing.Conclusion The baicalein-load-ed double network hydrogel exhibits promising potential in the enhancement of wound healing processes.
关键词
黄芩素/水凝胶/抑菌作用/创面愈合Key words
baicalein/hydrogel/antibacterial effect/wound healing引用本文复制引用
基金项目
国家自然科学基金面上项目(82073417)
广东省第二人民医院3D打印科研项目(3D-A20210051)
出版年
2024
NETL
NSTL
万方数据