Protection effect of irisin on brain tissue of ischaemic hypoxia mice
Objective To study the protection effect of irisin activating the nuclearfactor ery-throid-2-related factor-2(Nrf-2)/anti-oxidant response element(ARE)signaling pathway on brain injury in mice with ischaemic hypoxia.Methods Thirty-two 7-week-old male C57BL/6N clean grade mice were divided into sham surgery group,model group,low-dose irisin group,and high-dose irisin group(n=8).Rat model of ischaemic hypoxia was constructed u-sing ligation of the common carotid artery combined with hypoxia,while the control group did not receive ligation or hypoxia treatment.The water maze experiment was used to detect the cognitive function of mice;HE staining was used to observe pathological changes in brain tis-sue;Transmission electron microscopy was used to observe neuronal structural changes;Com-mercial kits were used to measure catalase(CAT),super oxide dimutese(SOD)and malondial-dehyde(MDA)levels in brain tissues;Western blot method was used to detect the protein ex-pression of Nrf-2 and heme oxygenase-1(HO-1).Results Compared with the model group,mice in irisin group had a significantly shorter average escape latency and an increased average number of crossing platforms in the water maze test(P<0.05);Tissue section staining showed that a large number of neuronal cell cytoplasmic vacuolization was found in the cerebral cortex area of the model group mice,with neurons arranged in a network,resulting in extensive neuro-nal damage,the degree of neuronal edema and degeneration gradually improved in the low and high dose groups of irisin;The results of transmission electron microscopy showed that irisin im-proved neuronal structural abnormalities;Compared mice in the sham operated group,the CAT and SOD activities in the brain tissue of the model group mice were significantly reduced,and the MDA content was significantly increased,irisin increased the CAT and SOD activities in the mouse brain tissue and decreased the MDA content(P<0.05);Western blot results showed that compared with the sham operated group mice,the model group mice had significantly lower levels of Nrf-2 and HO-1,while irisin increased the levels of Nrf-2 and HO-1 in the mouse brain tissue(P<0.05).Conclusion Irisin may improve cognitive impairment in mice with ischaemic hypoxia by regulating oxidative stress in the brain.
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