Study on the treatment of clear cell renal cell carcinoma based on nanoprobe fluorescence imaging
Objective To improve the preoperative diagnosis and treatment of renal cancer,a novel nanoprobes targeting clear cell renal cell carcinoma(ccRCC)clear cell renal cell carcino-ma targeting system(CTS))is developed to evaluate the targeting ability,fluorescence effect,and early diagnostic value of CTS in fluorescence molecular imaging of clear cell renal cell car-cinoma.Methods The nanoprobes CTS were constructed and co-cultured with human clear cell renal cell carcinoma cell lines 786-O and Caki-1.The targeting situation of CTS and the receptors was observed using a laser confocal microscope.Subsequently,a subcutaneous trans-plantation tumor model of clear cell renal cell carcinoma was established in black mice.After tail vein injection of CTS,in vivo fluorescence molecular imaging was performed to observe the imaging effect of CTS and analyze the fluorescence signal intensity at the tumor site.Finally,tumor tissues were collected from the mice for immunohistoghemistry(IHC)and immunofluores-cence staining to examine the targeting effect of CTS.Results Under laser confocal microsco-py,it was observed that CTS and the receptors co-localized well.The in vivo fluorescence mo-lecular imaging results showed that the probe concentrated in the subcutaneous tumor site of the experimental mice,demonstrating that CTS had good targeting labeling and signal-to-noise rati-o.Finally,tumor tissues were collected from the mice for IHC and frozen section immunofluo-rescence staining,and the results indicated that carbonic anhydrase IX(CAIX)was overex-pressed in ccRCC tissues and that CTS could effectively target CAIX.Conclusion CTS has a good ability to target ccRCC cells and imaging effects,and it can achieve early tumor diagnosis and guide tumor resection through fluorescence molecular imaging technology.
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