In order to investigate the effects of sodium diethyldithiocarbamate(DIECA)on wheat male fertility,the ther-mo-sensitive genic male sterile wheat BNS366 and its near-isogenic line Zhengmai366 were used as materials.The two materials were sown under conditons of normal sowing in autumn(2021-10-13)and late sowing(2021-12-01),respectively.In the spring of 2022,indirect enzyme-linked immunosorbent assays method was used to endogenous methyl jasmonate(MeJA)content in young panicles and anthers from the differentiation stage of pistil and stamen primordium to anthesis,and the wheat was sprayed with 7 different concentrations(0(water/CK)、0.1、1、5、10、20 and 30 mmol·L-1)of DIECA solution.I2-KI method was used to detect the frequency of fertile pollen grains at anthesis stage.National and international methods were used to deter-mine the self-seeds rate.The results showed that under the condition of normal sowing in autumn,after the pollen mother cell stage,there was stimulating growth in the content of endogenous MeJA in Zhengmai366,however,there was shortage in BNS366.Under the condition of normal sowing in autumn,sprayed with 1 mmol·L-1 DIECA and 5 mmol·L-1 DIECA the self-seeds rate of Zhengmai366 were decreased which meant the male fertility of Zhengmai366 was decreased,and the treatment of 5 mmol·L-1 DIECA had stronger effect on it,i.e.,the self-seeds rates by national and international methods were reduced by 39.82％and 44.68％respectively compared with the control group,the frequency of fertile pollen grains and the self-seeds rates by national and international methods of Zhengmai366 and BNS366 under other DIECA treatments were not significantly different from the control.It was probable that DIECA inhibited the stimulating growth above mentioned,that affected the an-ther development and dehiscence regularly that decreased the male fertility of Zhengmai366,and the endogenous MeJA might involved the occurrence of male sterility in BNS366.
万方数据
维普
