Expression of circPDE3B in esophageal squamous cell carcinoma tissue and its effect on the biological behavior of cancer cells
Aim:To explore the expression of circPDE3B in esophageal squamous cell carcinoma(ESCC)tissue and its effect on the proliferation,migration and invasion of ESCC.Methods:A total of 30 cases of ESCC and corresponding normal esophageal tissues adjacent to cancer were collected.qRT-PCR was used to detect the expression of circPDE3B.KYSE150 cells were divided and transfected with si-NC,si-circPDE3B#1,si-circPDE3B#1 + inhibitor NC,si-circPDE3B#1 + miR-1294 inhibitor,respectively.CCK-8,clone formation assay,wound healing assay,Transwell migration and invasion assays were used to detect the proliferation,migration and invasion abilities of ESCC cells.Dual-luciferase reporting assay was used to detect the targeting relationship between circPDE3B and miR-1294.Results:The expression of circPDE3B in ESCC tis-sue was higher than that in normal esophageal tissue adjacent to cancer(P<0.05).Compared with the si-NC group,the proliferation of KYSE150 cells was inhibited in si-circPDE3B#1 and si-circPDE3B#1 +inhibitor NC groups,while the num-ber of clone formation,migration cells,and invasion cells decreased,and the scar healing efficiency reduced(P<0.05).miR-1294 inhibitor could partially reverse the above changes(P<0.05).The results of the dual-luciferase reporting assay showed there was a target relationship between circPDE3B and miR-1294 mimic.Conclusion:The expression of circPDE3B is upregulated in ESCC tissue,and circPDE3B can competitively bind to miR-1294 and affect the proliferation,migration,and invasion abilities of ESCC cells.
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维普
