Effect of sulforaphane-rich broccoli extract on intestinal microbiota and the activation of peripheral blood CD8+ T lymphocytes of the mouse
Aim:To explore the impact of broccoli extract with sulforaphane(SFN)on the mouse intestinal microbiota and the activation of peripheral blood CD8+T lymphocytes.Methods:A total of 15 C57BL/6N mice were randomly alloca-ted into blank control(CON)group,negative control(NC)group,and SFN group,with 5 mice in each group.The mice were administered distilled water,broccoli extract without SFN,or broccoli extract with0.3 g/L SFN by gavage,respectively,for 4 weeks consecutively.Body weight was measured every 2 days.Peripheral blood was collected at the end of the experiment to assess the percentage of activated CD8+T lymphocytes and the levels of IL-2 and TNF-α in the CD8+T lymphocytes.Fecal samples were collected for analysis of the intestinal microbiota.Results:There was no statistical difference in body weight a-mong the three groups(P>0.05).The percentage of activated CD8+T lymphocytes and the levels of IL-2 and TNF-α in the CD8+T lymphocytes were higher in the SFN group than those in the CON and NC groups(P<0.05).The composition of the intestinal microbiota differed among the groups,with higher relative abundances of Verrucomicrobia and Akkermansia in the SFN group compared with the CON and NC groups(P<0.05).The relative abundance of Akkermansia in the intestine was positively correlated with the levels of IL-2 and TNF-α(r =0.934 and0.664,P<0.05).Conclusion:Long-term con-tinuous consumption of broccoli extract rich in SFN can increase the relative abundance of beneficial bacteria such as Akker-mansia,in the mouse intestine,and enhance the function of peripheral blood CD8+T lymphocytes.
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