Effects of arginine on Streptococcus mutans biofilm and remineralization of artificial cementum lesions:in vitro study
Aim:To observe the effects of arginine on Streptococcus mutans(S.mutans)biofilm and remineralization of cementum,and explore the clinical effects and feasibility of arginine on the prevention of root caries.Methods:Different concentrations of arginine were added to the culture medium to detect the effect of arginine on S.mutans biofilm growth.The culture medium without arginine served as control group.After 24 hours,pH determination and crystal violet staining were used to detect the effect of arginine on S.mutans biofilm growth.Cementum blocks were prepared from isolated human per-manent premolars and made cementum root caries model.They were divided into 4 groups:control group(artificial saliva im-mersion)and 3 experimental groups(7.5,15.0,and 30.0 g/L arginine solution immersion).After treatment with gradient arginine solutions and 20-day remineralization experiment,physical and chemical compositions of cementum blocks were de-tected by scanning electronic microscope and energy dispersive spectrometer.Results:Compared with control group,argi-nine treatment showed obvious inhibitory effect on S.mutans biofilm(P<0.001),the surface structure of the root cementum was denser and the contents of Ca and P increased(P<0.001),and compared with low concentration arginine,high con-centration arginine had more significant effects on inhibition of biofilm and remineralization of root caries.Conclusion:Argi-nine could inhibit S.mutans biofilm formation and promote root caries cementum remineralization,which has significant effect on the prevention and early treatment of root caries.
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