首页|BHMT基因rs3733890和rs7356530多态性与妊娠糖尿病患病风险的关系

BHMT基因rs3733890和rs7356530多态性与妊娠糖尿病患病风险的关系

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨甜菜碱同型半胱氨酸S甲基转移酶(BHMT)基因rs3733890 和rs7356530 多态性与妊娠糖尿病(GDM)患病风险的关系.方法:选取 2019 年 12 月至 2022 年 4 月在天津医科大学朱宪彝纪念医院做孕检、汉族、年龄21～40 岁、妊娠24～28 周的GDM孕妇80 例,均经OGTT试验确诊.选择民族、年龄、孕周及孕前BMI相匹配的60 名同期健康孕妇作对照.采集外周血,ELISA法检测血清Hcy、叶酸和维生素B12 水平,Sanger测序法检测BHMT rs3733890 和rs7356530 多态性.采用Logistic回归分析GDM与两个多态性的关联.结果:GDM组血清Hcy和叶酸水平高于对照组(P<0.05).GDM组BHMT rs3733890 A等位基因频率高于对照组,GA和AA基因型频率高于对照组(P<0.05).两组BHMT rs7356530 等位基因和基因型频率差异无统计学意义(P>0.05).Logis-tic回归分析结果显示,调整了年龄、孕前BMI、叶酸和维生素B12 后,rs3733890 A等位基因与GDM患病风险增加有关,OR(95%CI)为2.624(1.254～5.494).结论:BHMT rs3733890 的A等位基因与GDM患病风险增加有关.
Association between BHMT rs3733890,rs7356530 polymorphism and the risk of gestational diabetes mellitus
Aim:To investigate the association between betaine homocysteine S-methyltransferase(BHMT)gene rs3733890,rs7356530 polymorphism and the risk of gestational diabetes(GDM).Methods:A total of 80 GDM pregnant women of Han nationality,aged 21-40 years,with gestational weeks of 24 to 28 from Chu Hsien-I Memorial Hospital of Tianjin Medical University from December 2019 to April 2022 were recruited.GDM was diagnosed through OGTT test.The control group consisted of 60 heathy pregnant women matched with GDM group in nationality,age,gestational age and BMI before pregnancy.The peripheral blood samples were collected,and serum Hcy,folate,and VitB12 levels were detected by ELISA,and BHMT rs3733890 and rs7356530 polymorphism was detected by Sanger sequencing.Logistic regression was used to estimate the association between BHMT rs3733890,rs7356530 polymorphism and GDM.Results:The levels of ser-um Hcy and folate in the GDM group were higher than those in the control group(P<0.05).The frequency of BHMT rs3733890 A allele in the GDM group was higher than that in the control group,while the frequency of GA +AA genotype was higher than that in the control group(P<0.05).There was no significant difference in the distribution of alleles or gen-otypes of BHMT rs7356530 between the 2 groups(P>0.05).Logistic regression result showed that after adjusting for age,pre-pregnancy BMI,folate and VitB12,rs3733890 A allele was associated with increase risk of GDM,OR(95%CI)of 2.624(1.254-5.494).Conclusion:A allele of BHMT rs3733890 may be associated with increased risk of GDM.

betaine homocysteine S-methyltransferasegestational diabetes mellitushomocysteine

王雅茹、刘蕊、张盈莹、李冲、张诗田、屈恩帅、牛文彦

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天津医科大学朱宪彝纪念医院检验科

天津市内分泌研究所

国家卫健委激素与发育重点实验室

天津市代谢性疾病重点实验室 天津 300134

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甜菜碱同型半胱氨酸S甲基转移酶 妊娠糖尿病 同型半胱氨酸

国家自然科学基金天津医科大学朱宪彝纪念医院科研项目天津医科大学朱宪彝纪念医院科研项目天津医科大学朱宪彝纪念医院科研项目天津市医学重点学科(专科)建设项目

818705472019ZDKF052019ZDKF022019ZDKF16TJYXZDXK-032A

2024

10.13705/j.issn.1671-6825.2023.03.022

郑州大学学报(医学版)
郑州大学

郑州大学学报(医学版)

CSTPCD北大核心
影响因子：1.246
ISSN：1671-6825
年,卷(期)：2024.59(2)
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