GPR176 mRNA与胃癌预后及免疫治疗反应关系的生物信息学分析
Bioinformatics analysis on the relationship of GPR176 mRNA with prog-nosis of gastric cancer and response to immunotherapy
朱怡臻 1王迎 2汤有才3
作者信息
- 1. 郑州大学第五附属医院胃肠甲状腺外科 郑州 450052
- 2. 郑州大学第五附属医院输血科 郑州 450052
- 3. 郑州大学第五附属医院儿科 郑州 450052;河南省康复医学重点实验室 郑州 450052;河南省慢性肝损伤国际联合实验室 郑州 450052;郑州市代谢相关脂肪性肝病重点实验室 郑州 450052;河南省慢性肝损伤杰出外籍科学家工作室 郑州 450052
- 折叠
摘要
目的:基于生物信息学技术探讨G蛋白偶联受体176(GPR176)在胃癌中的表达水平,挖掘其预测胃癌预后和免疫检查点阻断剂应答的潜力.方法:利用R软件比较TCGA-STAD和GEO数据集中胃癌组织和正常胃组织中GPR176 mRNA的表达,调取Human Protein Atlas中免疫组化图谱,比较胃癌组织和正常胃组织中GPR176 蛋白的表达.利用TCGA-STAD数据集分析不同临床特征胃癌患者GPR176 mRNA表达的差异.利用TCGA-STAD和GEO数据集分析GPR176 mRNA表达水平预测胃癌预后及免疫检查点阻断剂应答的价值.结果:与正常胃组织比较,胃癌组织中GPR176 mRNA和蛋白表达水平均上调,且与胃癌患者的不良临床特征有关.GPR176 mRNA高表达患者总生存期短于低表达者;ROC曲线分析表明,GPR176 mRNA对胃癌 1、3、5 a生存率具有较高的预测价值.GPR176 mRNA高表达患者免疫细胞(未激活NK细胞和M2 巨噬细胞)的浸润丰度高,免疫表型评分低.结论:GPR176 在胃癌组织中高表达,可作为潜在的预测胃癌预后和免疫检查点阻断剂应答的新型分子标志物.
Abstract
Aim:To investigate the expression level of G protein coupled-receptor(GPR176)in gastric cancer by bioinformatics,and explore its potential in predicting the prognosis and immune checkpoint blocker response in patients with gastric cancer.Methods:R software was used to analyze the expression difference of GPR176 mRNA in gastric cancer and normal gastric tissue using TCGA-STAD data set and GEO data set,and immunohistochemical image of Human Protein Atlas was used to analyze the expression of GPR176 protein in gastric cancer and normal gastric tissue.The expression of GPR176 mRNA in gastric cancer patients with different clinical features was compared using TCGA-STAD data set.The value of GPR176 mRNA in predicting prognosis of gastric cancer and immune checkpoint blocker response was explored using the TCGA-STAD data set and GEO data set.Results:Both mRNA and protein expressions of GPR176 were significantly up-reg-ulated in gastric cancer tissue,which was associated with adverse clinical features of patients with gastric cancer.Patients with higher GPR176 mRNA had shorter overall survival.ROC curve analysis showed that GPR176 mRNA was of some value in predicting the 1,3,5 year survival of gastric cancer.The gastric cancer patients with higher GPR176 mRNA had higher infiltration abundance in resting NK cells and macrophages M2,and lower immunophenotypic scores.Conclusion:GPR176 is highly expressed in gastric cancer tissue.It could be used as a potential novel molecular marker for predicting prognosis of gastric cancer and immune checkpoint blocker response.
关键词
G蛋白偶联受体176/胃癌/预后/免疫检查点阻断剂/生物信息学分析Key words
GPR176/gastric cancer/prognosis/immune checkpoint blocker/bioinformatics analysis引用本文复制引用
基金项目
国家自然科学基金面上项目(NSFC31471330)
国家重点研发计划(2020YFC2009006)
郑州大学学科重点专项(XKZDQY202001)
出版年
2024
国家科技期刊平台
NSTL
万方数据