木瓜提取物MGE30OA对吲哚美辛诱导的大鼠胃黏膜损伤的保护作用
Protective effects of MGE30OA extract from Chaenomeles speciosa on in-domethacin-induced gastric mucosal lesion in rats
彭校 1王晓 1李洁 1吴梦瑶 1赵秋瑟 1石孟琼 2何毓敏 2徐道祥 3余惠凡 4张继红5
作者信息
- 1. 天然产物研究与利用湖北省重点实验室 湖北宜昌 443002;三峡大学生物与制药学院 湖北宜昌 443002
- 2. 三峡大学医学院 湖北宜昌 443002
- 3. 温州市第七人民医院临床心理科 浙江温州 325000
- 4. 武当地方中医药研究重点实验室 湖北十堰 442000
- 5. 三峡大学中医临床医学院 湖北宜昌 443002;湖北省功能性消化系统疾病中医临床医学研究中心 湖北宜昌 443002
- 折叠
摘要
目的:探究木瓜提取物MGE30OA对吲哚美辛诱导的大鼠胃黏膜损伤的保护作用及其可能的机制.方法:将SD大鼠随机分为正常组,模型组,MGE30OA低、中、高剂量(25、50、100 mg/kg)组,每组5 只.模型组灌胃 4 mg/kg吲哚美辛;MGE30OA各剂量组灌胃吲哚美辛0.5h后,灌胃相应剂量的MGE30OA;1 次/d,持续14 d.末次给药后取大鼠胃黏膜组织,HE染色观察组织学表现,Western blot法检测胃组织中PI3K/AKT信号通路相关蛋白和MAPK3 蛋白的表达;取全血,ELISA法检测血清TNF-α、IL-1β、IL-6、TGF-β1、IL-4、IL-10、COX-1、COX-2、PGE2 水平.结果:模型组大鼠胃黏膜损伤明显,MGE30OA各剂量组胃黏膜损伤较模型组减轻.与正常组比较,模型组大鼠血清中促炎因子IL-6、IL-1β、TNF-α水平升高,抗炎因子 TGF-β1、IL-4、IL-10 水平降低,PGE2 和 COX-1 水平降低,COX-2 水平升高(P<0.05);胃组织中磷酸化(p-)PI3K/PI3K 和 p-AKT/AKT 降低,MAPK3 表达水平升高(P<0.05).与模型组比较,MGE30OA各剂量组血清IL-6、IL-1β、TNF-α水平降低,IL-4、IL-10、TGF-β1 水平升高(P<0.05);COX-2 水平降低,COX-1 及PGE2 水平升高(P<0.05);p-PI3K/PI3K与p-AKT/AKT升高,MAPK3 表达下调(P<0.05).结论:MGE30OA对吲哚美辛诱导的大鼠胃黏膜损伤有较好的保护作用,其作用机制可能与调节PI3K/AKT、MAPK信号通路有关.
Abstract
Aim:To investigate the protective effects of MGE30OA extracted from Chaenomeles speciosa on indometha-cin-induced gastric mucosal lesion in rats and the possible mechanism.Methods:SD rats were randomly allocated into nor-mal group,model group,and MGE30OA low,medium and high dose(25,50 and 100 mg/kg)groups.The model group was given 4 mg/kg indomethacin by gavage;the MGE30OA low,medium and high dose groups were first given indomethacin by gavage and then given corresponding dose of MGE30OA after 0.5 hour;gavage was administered once a day for 14 days.Af-ter the last administration,stomachs of these rats were taken,HE staining was performed to observe the histological manifes-tations of gastric mucosa,and the expressions of PI3K/AKT-related protein and MAPK3 in gastric tissues were detected by Western blot;whole blood was taken,and serum levels of TNF-α,IL-1β,IL-6,TGF-β1,IL-4,IL-10,COX-1,COX-2 and PGE2 were detected by ELISA.Results:The gastric mucosal lesion in the model group was obvious,but in each dose group of MGE30OA was significantly reduced.Compared with the normal group,the serum levels of pro-inflammatory factors IL-6,IL-1β and TNF-α increased,and the levels of anti-inflammatory factors TGF-β1,IL-4 and IL-10 decreased,and the levels of PGE2 and COX-1 dropped and COX-2 rose in the model group(P<0.05).p-PI3K/PI3K and p-AKT/AKT in gastric tissue were reduced and MAPK3 expression was upregulated(P<0.05).Compared with the model group,serum IL-6,IL-1β and TNF-α levels were declined,IL-4,IL-10 and TGF-β1 levels were augmented;COX-2 level fell,COX-1 and PGE2 levels grew;p-PI3K/PI3K and p-AKT/AKT were higher and MAPK3 expression was lower in each dose group of MGE30OA(P<0.05).Conclusion:MGE30OA has good protective effects on indomethacin-induced gastric mucosal lesion in rats,and the mechanism may be related to the regulation of PI3K/AKT and MAPK signaling pathway.
关键词
木瓜提取物/胃黏膜损伤/炎症因子/PI3K/AKT信号通路/MAPK信号通路/大鼠Key words
extract from Chaenomeles speciosa/gastric mucosal lesion/inflammatory factor/PI3K/AKT signaling path-way/MAPK signaling pathway/rat引用本文复制引用
基金项目
湖北省武当特色中医药研究重点实验室开放基金(WDCM2022010)
湖北省科技厅自然科学研究项目(2022CFB357)
湖北省科技厅自然科学研究项目(2022CFB427)
出版年
2024
国家科技期刊平台
NSTL
万方数据