Effect of miR-34c-5p targeting poFUT1 on placental angiogenesis
Aim:To investigate the targeting relationship between miR-34c-5p and poFUT1 and the effcet on placental angiogenesis.Methods:The binding sites between miR-34c-5p and poFUT1 were predicted by ENCORI database and veri-fied by dual luciferase reporter assay.A total of 20 pregnant women with fetal growth restriction(FGR)from the Third Affili-ated Hospital of Zhengzhou University from April to October 2023 were randomly selected,with 20 normal pregnant women during the same period serving as control.The expressions of miR-34c-5p and poFUT1 mRNA in placental tissue were de-tected using quantitative PCR.Human umbilic vein endothelial cells were transfected with miR-34c-5p mimic NC,miR-34c-5p mimic,miR-34c-5p inhibitor NC,miR-34c-5p inhibitor,si-poFUT1 NC,si-poFUT1,si-poFUT1+miR-34c-5p inhibitor,respectively,and cell proliferation,invasion and tube formation were verified using the CCK-8 assay,transwell assay,and tube formation experiment.Results:The expression of miR-34c-5p was(1.57±0.39),(1.09±0.18),and that of po-FUT1 mRNA was(0.51±0.17),(1.06±0.13)in FGR and normal placental tissue;compared with control group,the ex-pression of miR-34c-5p of FGR group was higher,and that of poFUT1 mRNA was lower(P<0.05).The number of invasive cells in miR-34c-5p mimic group was less,and tube formation capacity and the cell proliferative activity were lower than those of mimic NC group(P<0.05).The number of invasive cells in miR-34c-5p inhibitor group was more,and tube forma-tion capacity and the cell proliferative activity were higher than those of inhibitor NC group(P<0.05).Compared with si-poFUT1 NC group,the number of invasive cells in si-poFUT1 group was less,and tube formation capacity and the cell prolif-erative activity were lower(P<0.05),while the changes mentioned above in si-poFUT1+miR-34c-5p inhibitor group were reversed partly(P<0.05).Conclusion:MiR-34c-5p could regulate vascular endothelial cell function by poFUT1,thereby affecting placenta angiogenesis,and involve in FGR generation.
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