Efficiency of VavCre-mediated fluorescent reporter system for microglia labeling in mice
Aim:To investigate the efficiency of VavCre-mediated fluorescent reporter system for microglia labeling in mice.Methods:Male VavCie/Rosa26REYFPfl/fl mice were crossed with female C57BL/6 mice to obtain VavCre/Ro-sa26REYFPfl/-and Rosa26REYFPfl/-genotyped filial generation.The time point at which vaginal plug was found in female mice was recorded as E0.5,flow cytometry was performed to detect the expression of EYFP in mouse embryos of E7.5-E8.25,and in microglia from E10-E10.5,E12-E12.5,E14-E14.5 embryos,and neonatal mice.Results:The positive rates of EYFP in microglia from VavCre/Rosa26REYFPfl-E10-E10.5,E12-E12.5,E14-E14.5 embryos,and neonatal mice were significantly higher than those from Rosa26REYFPfl/-filial generation(P＜0.001),and all were more than 95.000％.The positive rate of EYFP were(0.059±0.009)％in VavCre/Rosa26REYFPfl/-E7.5-E8.25 embryos.Con-clusion:VavCre-mediated fluorescent reporter system could stably and efficiently label microglia of mice,and be used to mo-nitor microglia development.
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