Aim:To explore the treatment mechanism of Shouhuitongbian capsules(SHTB)against slow transit consti-pation.Methods:A total of 80 SD rats were allocated into control group,model group,moxapride group(MOS,10 mg/kg),and high-,low-dose(630,210 mg/kg)SHTB groups,with 16 in each group.Rats in the model group,MOS group,and SHTB groups were modeled with compound difenoxate tablets 10 mg/kg for 14 days;then MOS group and SHTB groups were ad-ministered by gavage at the corresponding dose,control group and model group were given physiological saline solution,once perday for 7 days.After the last administration,for 6 rats in each group,ink was given by gavage and the first appear-ance time of tarry stool,24 hours defecation number and the intestinal transit rate were recorded;the pathological changes of colon were observed by HE staining.For the other 10 rats in each group,16S rDNA was used to detect the structural changes of gut microbiota,and serum metabolites were analyzed by ultra-high performance liquid chromatography/tandem mass spectrometry.Results:Compared with those of the model group,the first appearance time of tarry stool of the high-dose SHTB group was shorter,24 hours defecation number and the intestinal transit rate increased,the damage of colon tissue was repaired,the ratio of Firmicutes/Bacteroides and the relative abundance of verrucobacteria were lower,but the relative a-bundance of Prevotella_9 and Prevotellaceae_UCG-001 were higher.Eight different metabolites were identified,which in-volved 7 metabolic pathways including histidine metabolism,β-alanine metabolism,primary bile acid biosynthesis,bile se-cretion,purine metabolism,primary bile acid biosynthesis conduction and its metabolic conduction.Conclusion:The mecha-nism of SHTB improving STC might be related to the regulation of gutmicrobiota and bile acid metabolic-related pathways.
维普
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