Aim:To explore the effects of circARF3/miR-338-3p on oxidized low-density lipoprotein(ox-LDL)-in-duced vascular endothelial cell injury and its possible mechanism.Methods:The dual luciferase reporter assay was used to detect the targeting relationship between circARF3 and miR-338-3p.Human umbilical vein endothelial cells(HUVEC)were allocated into 6 groups,blank control group(not treated),model group(treated with 50 mg/L ox-LDL for 24 hours),pcDNA group,pcDNA-circARF3 group,pcDNA-circARF3+miR-NC group,and pcDNA-circARF3+miR-338-3p group,and the lat-ter 4 groups were first transfected with the corresponding plasmids and then treated with ox-LDL for 24 hours.MTT method and Annexin V-FITC/PI were used to detect cell proliferation and apoptosis.Western blot was used to detect the expressions of Bax and Bcl-2.A kit was used to detect the activity of SOD,LDH and the level of MDA in culture supernatant.ELISA was used to detect the levels of IL-6 and TNF-α in culture supernatant.Results:circARF3 could targetedly bind and negatively regulate miR-338-3p.Compared with blank control group,the cell proliferation inhibition rate,apoptosis rate and the protein level of Bax were increased,while that of Bcl-2 decreased,the activity of SOD was decreased,the level of MDA and the ac-tivity of LDH were increased,and the levels of IL-6 and TNF-α were increased in the model group(P＜0.05).Compared with the model group and pcDNA group,the above changes in the pcDNA-circARF3 group were reversed(P＜0.05).Com-pared with the pcDNA-circARF3+miR-NC group,the above reversed changes were partially rescued in the pcDNA-cir-cARF3+miR-338-3p group(P＜0.05).Conclusion:Overexpression of circARF3 could alleviate the injury induced by ox-LDL by inhibiting the expression of miR-338-3p.
关键词
人脐静脉血管内皮细胞/circARF3/miR-338-3p/细胞增殖/凋亡/氧化应激/炎症
Key words
human umbilical vein endothelial cell/circARF3/miR-338-3p/cell proliferation/apoptosis/oxidative stress/inflammation
维普
万方数据