Effect of glutamine on malignant biological behaviors and SNX10/mTORC1 pathway proteins expression of thyroid cancer cells
Aim:To explore the effect of glutamine(Gln)on the malignant biological behaviors and the expression of SNX10/mTORC1 pathway proteins of thyroid cancer cells.Methods:Human undifferentiated thyroid cancer cell line C643 was cultured and treated with 0,1,2,4 mmol/L Gln,respectively,each concentration had 6 complex holes.After 24,48,72 hours culture,MTT assay was used to detect cell proliferative activity.After 24 hours culture,Annexin V/PI staining meth-od,scratch assay,Trans well assay,ATP assay were used to detect apoptosis,migration ability,invasion ability,and ATP-pro-ducing ability,RT-qPCR was used to detect the mRNA expressions of SNX1O,p-mTOR,mTORC1,and the expressions of SNX10,p-mTOR,mTORC1,Akt,p-Akt proteins were detected by Western blot.Results:After Gln culture,the apoptosis rate of C643 cells decreased,the proliferative activity,scratch healing rate,number of invasive cells and the concentration of ATP increased,the mRNA relative expressions of SNX10,p-mTOR and mTORC1 increased,and the protein expressions of SNX10,p-mTOR,mTORC1,Akt and p-Akt were up-regulated in a dose-dependent manner(P＜0.05).Conclusion:Gln may improve the malignant biological behaviors of thyroid cancer cells through activating SNX1O/mTORC1 pathway.
万方数据
维普
