Construction of lncRNA-related ceRNA regulatory network of diabetic kidney disease mouse based on high-throughput sequencing technology
Aim:To screen the differentially expressed long non-coding RNA(lncRNA)and microRNA(miRNA)in the kidney tissue of diabetic kidney disease(DKD)mice and construct a lncRNA-related competing endogenous RNA(ceR-NA)regulatory network.Methods:A total of 6 BKS-db/m male mice were selected as control group,and 6 BKS-db/db male mice were selected as model group to establish the DKD mouse model.The mice were sacrificed and their kidney tissue samples were collected for high-throughput sequencing.The differentially expressed lncRNAs and miRNAs between the 2 groups were screened using DESeq software.The target genes of differentially expressed miRNAs were predicted using Mi-randa software.A lncRNA-related ceRNA regulatory network was constructed,and GO functional enrichment analysis and KEGG signaling pathway enrichment analysis were performed.Results:A total of 1 495 differentially expressed lncRNAs and 72 differentially expressed miRNAs were screened.A lncRNA-related ceRNA network consisting of 23 lncRNAs such as MSTRG.7252.3,MSTRG.10465.2,MSTRG.16253.3,etc,23 miRNAs,and 2 mRNAs was successfully constructed.The GO functional enrichment analysis showed that the main categories involved biological processes,cellular components,and molecular functions.The KEGG signaling pathway enrichment analysis revealed that the pathways were primarily related to PPAR signaling pathway,hematopoietic cell lineage,cell adhesion molecules,TNF signaling pathway,etc.Conclusion:A ln-cRNA-related ceRNA regulatory network in DKD mice has been successfully constructed.
万方数据
维普
