Relationship between optimized sperm DNA fragmentation index and IVF-ET embryo quality and pregnancy outcomes
Aim:To evaluate the relationship between the sperm DNA fragmentation index(DFI)optimized by density gradient centrifugation combined with swim-up,and embryo quality and pregnancy outcomes of assisted reproduction in vitro fertilization-embryo transfer(IVF-ET).Methods:A retrospective analysis was conducted on 257 IVF-ET cycles performed at the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from April to December 2022,involving patients with tubal factor infertility.Semen parameters and sperm DFI were compared before and after optimiza-tion.Based on the optimized sperm DFI values,patients were allocated into high DFI group(DFI>5)and low DFI group(DFI≤5).The embryonic development and pregnancy outcome of the 2 groups were compared.According to the clinical pregnancy conditions,they were allocated into pregnant group and non-pregnant group,continuous pregnancy group and early miscarriage group,and the DFI of sperm was compared.Results:After semen optimization,the proportion of progressive mo-tile sperm and normal morphology sperm increased,and sperm DFI significantly decreased(P<0.001).The early miscar-riage rate in the high DFI group was significantly higher than that in the low DFI group(P<0.05)following sperm optimi-zation.In 105 clinical pregnancy cycles,the average DFI before and after sperm optimization was higher in the early miscar-riage group than that in the continuous pregnancy group(P<0.05).Conclusion:Density gradient centrifugation combined with swim-up is an effective method for semen preparation,improving the proportion of progressive motility and normal mor-phology of sperm while reducing sperm DFI.However,a high DFI after optimization may increase the risk of early miscar-riage after clinical pregnancy.
sperm DNA fragmentation indexdensity gradient centrifugation combined with swim-upIVF-ETembryo qualitypregnancy outcome
万方数据
维普
