Effects of activated macrophages on repair of optic nerve axotomy after lens injury of rat
Aim:To investigate the effects of activated macrophages on the regeneration of optic nerve axotomy after lens injury of rat.Methods:A total of 45 healthy adult Wistar rats were randomly allocated into 3 groups,15 in each group:normal control group(without any treatment),NC group(with optic nerve axotomy),NC+LP group(with optic nerve axoto-my firstly,and then lens injury).On the 7th,14th,21th day after operation,5 rats were executed,respectively,in each group,ED-1 expression and GAP-43 expression in retinal tissue were detected by immunohistochemistry and immunofluores-cence staining,respectively.Results:On the 7th,14th,21th day after operation,ED-1 positive cells were not detected in normal control group and NC group;while the number of ED-1 positively cells was(58.27±4.79),(45.39±5.13),(21.17±3.40)in NC+LP group,which was the highest at the7th day,and gradually decreased over time(P<0.001).On the 7th day,the expression of GAP-43 in retinal ganglion cell layer of NC group was higher than that of normal control group(P<0.05),and then decreased;the expression of GAP-43 RGC of NC+LP group was higher than that of NC group at all time points(P<0.05),and the expression was strongest on the 14th day after operation(P<0.05).Conclusion:The activation of macrophages could promote axonal regeneration of the optic nerve after lens injury.
万方数据
维普
