Changes of endogenous phytohormone contents in Platycladus orientalis roots after root-cutting
Contents of ABA, GA3, IAA, CTK in the root of 1/2 (A), 1/3 (B) and 1/4 (C) of main root-cutting seedling of one-year Platycladus orientalis were measured compared with the intact seedling (CK). The results showed that the content of ABA incresed quickly one day after the start of the experiment, and the more cut, the more increased. Then, it gradually decreased, which might be responsible for the restoration of root system. The content of CTK decreased quickly one day ( 1/2 rootcutting) or four days ( 1/3 and 1/4 root-cutting) after the start of the experiment, and then gradually increased. The more root was cut, the smaller difference was from the control, which is because the content of CTK in root is affected not only by the synthsis rate, but by the transport capacity to the shoot. After root-cutting, root IAA content gradually declined. On the other hand, a peak value of root IAA content emergenced 1 d after cutting, and this might weaken the inhibition to root growth from the increase of root ABA. The root GA3 content was not affected by root-cutting significantly, but gradually declined during the subsequent days of the experiment with similar decrcase rates.
root-cuttingendogenous phytohormonePlatycladus orientalis seedlingroot system