Gene knockout of TaMBD9 and its effects on leaf angle in wheat
[Objective]To obtain gene knockout materials of TaMBD9 and explore its biological func-tion in the regulation of growth and development in wheat.[Method]The expression pattern of TaMBD9 was analyzed by qRT-PCR and the gene knockout wheat materials were created by CRISPR-Cas9-medi-ated gene editing technology.[Result]The expression analysis showed that TaMBD9 was differentially expressed among wheat tissues with the highest expression level in leaves,and its expression in leaves fluctuated with the development process.There were some differences in the expression levels among three homoeologs,and the expression level of TaMBD9D was higher than that of the other two homoeo-logs in detected tissues.The homozygous wheat mutants were obtained by sucessfully knocking out all three homoeologs of TaMBD9.Phenotypic analysis demonstrated that the knockout of TaMBD9 resulted in a significant increase of leaf angle in wheat.[Conclusion]The TaMBD9 plays an important role in the regulation of leaf angle in wheat,and the obtained gene knockout lines of TaMBD9 provides new materials for further exploring the molecular regulatory mechanisms of leaf angle in wheat.
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