Kiwifruit canker is the main bacterial disease caused by Pseudomonas syringae pv.actinidiae(Psa).In this work,Psa strains were isolated and identified from diseased kiwifruit plants in differ-ent orchards for pathogenicity analysis.The results showed that six strains with P.syringae like morphological characteristics were obtained.After polymerase chain reaction(PCR)identification and sequence alignment,the isolates were identified as Psa.The inoculation of these strains in kiwifruit cultivar Hongyang exhibited their different pathogenic capabilities.ScYbH2,ScDjyH2 and ScYbH1 caused canker disease symptoms in inoculated plants,while ScMbJ1,ScDjyH3 and ScYaH2 did not possess serious pathogenicity.Moreover,the hypersensitivity reactions shown in tobacco leaves indi-cated different plant immunity activation elicited by the Psa isolates.In this study,isolated kiwifruit bacterial canker strains were obtained,and their phenotypic difference will provide a basis for further genetic analyses of pathogenic genes.
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维普
万方数据
