Aiming at the influence of the position change of the isolation layer on the vertical dynamic responses of high-rise structure under vertical earthquake action,the vertical dynamic responses of the five kinds of inter-story isolation structures under three kinds of vertical seismic waves are studied in this paper.Taking the Benchmark twenty-story steel structure model of the international platform as the standard model,the finite element numerical models of isolation structures of the first,third,fifth,seventh and ninth stories are established by ANSYS software.Then,the vertical dynamic re-sponses of the five kinds of inter-story isolation structures under vertical ElCentro wave,vertical Taft wave and vertical Tianjin wave,such as vertical story displacement,story displacement angle,vertical story acceleration and axial force of the base,are compared and analyzed.The results show that under the action of vertical earthquake,the control effect on vertical story displacement and story displace-ment angle is poor,and even they will increase,and the control effect on the axial force of the base is small;the vertical story acceleration and the axial force of the base increase gradually with the rise of the isolation layer,but when the isolation layer is located in the ninth story,the vertical story acceler-ation and the axial force of the base decrease to some extent compared with those with the isolation layer located in the seventh story.The research results of this paper can be used in structural seismic design of high-rise buildings considering vertical earthquake action.