In order to study the genetic relationship between the genes NAS2 and BZIP44 in the re-sponse of Arabidopsis thaliana to iron deficiency stress,35 S∶NAS2/WT transgenic plants were con-structed using wild type(WT)Arabidopsis thaliana as materials,and 35S∶NAS2/bzip44 transgenic plants were constructed using Arabidopsis thaliana mutant bzip44 as materials.The RNA of WT Ar-abidopsis thaliana was extracted,and the CDS region of NAS2 was cloned with the inverted cDNA as a template.The target fragment after double enzyme digestion was ligated with the pART 27 plasmid of the same double enzyme digestion,and the resulting ligation product was transferred into E.coli DH5a competent cells.A positive single clone was obtained by polymerase chain reaction(PCR)and sequencing,and then the recombinant plasmid was transferred into Agrobacterium GV3101 competent cells to obtain a positive single clone.Then,the WT and homozygous Arabidopsis thaliana bzip44 mutant plants were infected with the floral dip method,respectively.The homozygous 35S:NAS2/WT and 35S∶NAS2/bzip44 transgenic plants were obtained through screening and identification.The results of this study provide reference for the genetic relationship of genes NAS2 and BZIP44 in the response to iron deficiency stress in Arabidopsis thaliana.
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