Optimal culture of an acid-tolerant Aspergillus strain and its effect on Cu2+removal
Bioremediation is one of the important means to deal with heavy metal pollution,and fungi have a good application prospect in bioremediation.A filamentous fungus was isolated from wastewater from a mine in Ma'anshan City,Anhui Province and its effect on the removal of Cu2+was studied.It was identified as Aspergillus sp.MF1 by morphological analysis and 18SrDNA sequence analysis.The results showed that the maximum biomass was obtained when the pH value was 3.5,the carbon source was glucose,the nitrogen source was yeast,and the carbon to nitrogen ratio was 10.0.The maximum tolerance mass rate of Cu2+was 770.18 mg/g,and Cu2+could be effectively re-moved.When ρ0(Cu2+)was 50 and 100 mg/L,the Cu2+removal rates of Aspergillus sp.MF1 could reach 78.49％and 64.63％,respectively,and wmax(Cu2+)was 123.99 mg/g.In the physiological re-sponse of Aspergillus sp.MF1 to Cu2+stress,the role of proteins increased with the increase ofρ(Cu2+)and decreased with the increase of time.Aspergillus sp.MF1 has the ability to remove Cu2+in a strongly acidic environment,and this study can provide reference for the treatment of Cu2+pollu-tion by fungi in extreme environments.
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