一株耐酸曲霉的优化培养及其对Cu2+的去除作用
Optimal culture of an acid-tolerant Aspergillus strain and its effect on Cu2+removal
贾亚文 1王进 1王绍平 2王广成 2李伟 2岳正波1
作者信息
- 1. 合肥工业大学资源与环境工程学院,安徽合肥 230009
- 2. 安徽马钢矿业资源集团南山矿业有限公司,安徽马鞍山 243000
- 折叠
摘要
生物修复是处理重金属污染的重要手段之一,真菌在生物修复中具有良好的应用前景.文章从安徽省马鞍山市某矿山废水中分离出一株丝状真菌并研究其对Cu2+的去除作用.经形态学及18SrDNA序列分析鉴定为曲霉,命名Aspergillus sp.MF1.研究表明:在pH值为3.5条件下,碳源为葡萄糖、氮源为酵母、碳氮比为10.0时有最大生物量.菌体对Cu2+最大耐受质量比为770.18 mg/g,并能有效地去除Cu2+.ρ0(Cu2+)为 50、100 mg/L 时,Aspergillus sp.MF1 对 Cu2+的去除率分别可以达到 78.49％和 64.63％,且wmax(Cu2+)=123.99 mg/g.在Aspergillus sp.MF1抗Cu2+胁迫的生理响应过程中,蛋白质的作用随着ρ(Cu2+)增大而增加,随时间的增长而减小.菌株Aspergillus sp.MF1具有在强酸性环境下去除Cu2+的能力,该研究可为真菌在极端环境下治理Cu2+污染提供一定的参考.
Abstract
Bioremediation is one of the important means to deal with heavy metal pollution,and fungi have a good application prospect in bioremediation.A filamentous fungus was isolated from wastewater from a mine in Ma'anshan City,Anhui Province and its effect on the removal of Cu2+was studied.It was identified as Aspergillus sp.MF1 by morphological analysis and 18SrDNA sequence analysis.The results showed that the maximum biomass was obtained when the pH value was 3.5,the carbon source was glucose,the nitrogen source was yeast,and the carbon to nitrogen ratio was 10.0.The maximum tolerance mass rate of Cu2+was 770.18 mg/g,and Cu2+could be effectively re-moved.When ρ0(Cu2+)was 50 and 100 mg/L,the Cu2+removal rates of Aspergillus sp.MF1 could reach 78.49％and 64.63％,respectively,and wmax(Cu2+)was 123.99 mg/g.In the physiological re-sponse of Aspergillus sp.MF1 to Cu2+stress,the role of proteins increased with the increase ofρ(Cu2+)and decreased with the increase of time.Aspergillus sp.MF1 has the ability to remove Cu2+in a strongly acidic environment,and this study can provide reference for the treatment of Cu2+pollu-tion by fungi in extreme environments.
关键词
丝状真菌/生长条件/重金属/生长胁迫Key words
filamentous fungi/growth conditions/heavy metals/growth stress引用本文复制引用
基金项目
国家自然科学基金区域创新发展联合基金安徽项目(U20A20325)
出版年
2024
NETL
NSTL
万方数据