丙二酸盐克罗诺杆菌rpoS基因突变株与回补株的构建
Construction of rpoS gene mutant and complement strains of Cronobacter malonaticus
姚悦 1叶应旺 1凌娜 1王洋 1李杨1
作者信息
- 1. 合肥工业大学食品与生物工程学院,安徽合肥 230601
- 折叠
摘要
丙二酸盐克罗诺杆菌(Cronobacter malonaticus)在面对环境胁迫时,通过自身调节机制适应压力.rpoS基因作为一种稳定期σ因子和压力应答过程中的主要调节因子,在细菌抵抗或耐受环境胁迫压力中发挥重要作用.文章运用red同源重组技术和质粒表达系统,构建C.malonaticus G362的rpoS基因缺失株ΔrpoS和回补菌株ΔrpoS-com,为研究丙二酸盐克罗诺杆菌中rpoS基因的功能提供遗传材料.生长曲线测定结果表明,rpoS基因缺失未对正常培养条件下细菌生长产生显著影响.
Abstract
Cronobacter malonaticus adapts to stress through self-regulation mechanism in the face of en-vironmental stress.As a stable phase a factor and a major regulator in the process of stress response,rpoS gene plays an important role in bacterial resistance or tolerance to environmental stress.In this paper,red homologous recombination technology and plasmid expression system were used to con-struct the rpoS gene deletion strain ΔrpoS and complement strain ΔrpoS-com of C.malonaticus G362,providing genetic materials for studying the function of rpoS gene in C.malonaticus.The results of growth curve measurement showed that rpoS gene deletion had no significant effect on bacterial growth under normal culture conditions.
关键词
丙二酸盐克诺罗杆菌/同源重组/质粒表达/rpoS基因Key words
Cronobacter malonaticus/homologous recombination/plasmid expression/rpoS gene引用本文复制引用
基金项目
国家自然科学基金(32102104)
国家自然科学基金(32230084)
安徽省自然科学基金杰出青年基金(2208085J11)
出版年
2024
NETL
NSTL
万方数据