Construction of rpoS gene mutant and complement strains of Cronobacter malonaticus
Cronobacter malonaticus adapts to stress through self-regulation mechanism in the face of en-vironmental stress.As a stable phase a factor and a major regulator in the process of stress response,rpoS gene plays an important role in bacterial resistance or tolerance to environmental stress.In this paper,red homologous recombination technology and plasmid expression system were used to con-struct the rpoS gene deletion strain ΔrpoS and complement strain ΔrpoS-com of C.malonaticus G362,providing genetic materials for studying the function of rpoS gene in C.malonaticus.The results of growth curve measurement showed that rpoS gene deletion had no significant effect on bacterial growth under normal culture conditions.
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