In this paper,the crude polysaccharide was isolated from the fruiting bodies of white Hyp-sizygus marmoreus by water extraction at boiling temperature.After purification by DEAE-Cellulose ion exchange chromatography,the homogenous polysaccharide fraction WHB3 was obtained.The re-sults of uronic acid determination showed that WHB3 contained no uronic acid.Monosaccharide com-position analysis showed that WHB3 contained only glucose and its relative molecular mass was deter-mined to be 2.3 × 106 Da.Structural characterization results indicated that the main chain of WHB3 was consisted of(1→ 6)-β-D-Glcp,part of which was connected with branched 1-α-D-Glcp at the O-3 position.The anti-tumor activity of WHB3 and its hydrolysate WHB3-S on HepG2 cells was investi-gated in vitro by establishing hepatocellular carcinoma cell model.The results showed that the inhibi-tory effects of WHB3 and WHB3-S on HepG2 cells were positively correlated with the polysaccharide mass concentration.The structure-activity relationship analysis showed that the inhibitory rates of WHB3 and WHB3-S on HepG2 cells were 38.18％and 35.88％when the polysaccharide mass con-centration reached 500 μg/mL.The inhibitory rates of WHB3-S were significantly lower than those of WHB3 at different concentrations.These results indicated that the loss of branched chain and the de-crease of molecular weight had significant effects on the anti-tumor activity of WHB3,and reduced the tumor inhibition activity of WHB3.The results of this study provide insight for revealing the potential connection between the chemical structure and biological activity of the white Hypsizygus marmoreus polysaccharides.
white Hypsizygus marmoreuspolysaccharidestructural characterizationanti-tumor ac-tivitystructure-activity relationship
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