Tomato(Solanum lycopersicum)has high nutritional value and is deeply loved by consumers.At present,tomato has become one of the widely planted cash crops.Ethylene response factor(ERF)is a kind of plant-specific transcription factor.ERF plays an important role in a variety of physiological processes,such as plant morphogenesis,stress response,maturation and senescence,hormone signal transduction and metabo-lite regulation.In this paper,taking the SlERF-H6(LOC101259323)gene of tomato as the research object,the specific region silencing sequence of SlERF-H6 gene was obtained by analyzing the sequence of SlERF-H6 gene.The virus-induced gene silencing(VIGS)vector of tomato SlERF-H6 was successfully constructed by homologous recombination technique.The purpose of this study is to provide a reference for further explo-ring the role of tomato SlERF-H6 gene in fruit ripening.
tomato(Solanum lycopersicum)virus-induced gene silencing(VIGS)SlERF-H6 genehomologous recombinationvector construction
维普
万方数据
