Iron(Fe)is an essential micronutrient for plant growth.Nowadays plants are widely affected by Fe deficiency stress,which seriously affects plant growth and reduces crop yield.Therefore,screening and cloning of Fe deficiency stress tolerance genes are of great scientific value and practical significance to solve agricultural production problems.In this study,Arabidopsis thaliana was used as experimental material and the promoter region of the WRKY13 gene was cloned by polymerase chain reaction(PCR).The ProWRKY13:GUS fusion expression vector was successfully constructed.The ProWRKY13:GUS vector was transformed into A rabidopsis by the floral dip method,and posi-tive transgenic plants were obtained after identification and analysis.The transgenic plants were sub-jected to GUS tissue staining induced by Fe deficiency.The results provide an important basis for studying the function and transcriptional changes of WRKY13 gene under Fe deficiency stress.
维普
万方数据