The mutant plants with cadmium(Cd)tolerance obtained by screening the mutant library may be involved in the regulation of plant Cd stress tolerance.In this paper,pART27-CTM2-GFP vector was constructed by homologous recombination method,and the recombinant plasmid of pART27-CTM2-GFP was transferred into A grobacterium GV3101 and then into the wild-type Ara-bidopsis plant by floral dip transformation.CTM2-GFP transgenic positive plants were obtained through resistance screening and polymerase chain reaction(PCR)identification,and homozygous transgenic plants were obtained through resistance isolation and identification.Finally,CTM2 gene expression was detected by qPCR to screen the best CTM2-GFP transgenic lines.In addition,by ob-serving the green fluorescent protein(GFP)fluorescence in CTM2-GFP transgenic plants,it was found that CTM2 was a cell membrane localization protein.The results provide genetic material sup-port for the in-depth study of the role of CTM2 gene in regulating the molecular mechanism of plant Cd tolerance.
维普
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