Study on the mechanism of pear peel anthocyanin synthesis pathway in response to pathogen infection
Anthocyanin is a common pigment in fruit,and its role in fruit response to pathogen infec-tion is unclear.In this paper,the wild type(WT)strains of Aspergillus niger(A.niger)and the re-lated strains with deletion of antioxidant enzymes ΔcpeA,ΔsodB and ΔsodC were used to infect Dang-shan pear and Starkrimson pear.Anthocyanin-rich Starkrimson pears were found to be highly resist-ant to A.niger.Therefore,the anthocyanins of Starkrimson pear were extracted,and Escherichia co-li(E.coli)and A.niger were treated with 0,5％and 10％anthocyanins,respectively,and it was found that 5％and 10％anthocyanins showed different inhibitory effects on both E.coli and A.ni-ger.The Starkrimson pears were infected by A.niger,and the expression changes of anthocyanin-re-lated genes in the infected lesions of pears were analyzed by RT-qPCR.The expression levels of Py-MYB10,PyMYB114,PybHLH3,PyDFR,PyANS and PyUFGT were up-regulated,indicating that pear fruit may inhibit the infection of the postharvest pathogenic fungus A.niger by up-regula-ting the anthocyanin synthesis pathway.
万方数据
维普
