合肥工业大学学报(自然科学版)2024,Vol.47Issue(12) :1675-1679.DOI:10.3969/j.issn.1003-5060.2024.12.014

梨果皮花青素合成通路响应病原菌侵染机制研究

Study on the mechanism of pear peel anthocyanin synthesis pathway in response to pathogen infection

马琳 赵玉琪 李闯 张华 胡康棣
合肥工业大学学报(自然科学版)2024,Vol.47Issue(12) :1675-1679.DOI:10.3969/j.issn.1003-5060.2024.12.014

梨果皮花青素合成通路响应病原菌侵染机制研究

Study on the mechanism of pear peel anthocyanin synthesis pathway in response to pathogen infection

马琳 1赵玉琪 1李闯 1张华 1胡康棣1
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作者信息

  • 1. 合肥工业大学食品与生物工程学院,安徽 合肥 230601
  • 折叠

摘要

花青素是果实中的常见色素,在果实应答病原菌侵染中的作用尚不清楚.文章利用黑曲霉的野生型菌株、抗氧化酶缺失相关菌株ΔcpeA、ΔsodB和ΔsodC侵染砀山酥梨和红茄梨,发现富含花青素的红茄梨对黑曲霉具有较高的抵抗力.因此,分别用体积分数为0、5%、10%红茄梨提取的花青素处理大肠杆菌和黑曲霉,结果发现体积分数为5%、10%的花青素对大肠杆菌和黑曲霉都有不同程度的抑制作用.利用黑曲霉侵染红茄梨,通过实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术,分析梨侵染病变部位与花青素相关的基因表达量变化,发现PyMYB10、PyMYB1 14、PybHLH3、PyDFR、PyANS和PyUFGT表达量上调,结果表明,梨果实可能通过上调花青素合成通路抑制采后病原真菌黑曲霉的侵染.

Abstract

Anthocyanin is a common pigment in fruit,and its role in fruit response to pathogen infec-tion is unclear.In this paper,the wild type(WT)strains of Aspergillus niger(A.niger)and the re-lated strains with deletion of antioxidant enzymes ΔcpeA,ΔsodB and ΔsodC were used to infect Dang-shan pear and Starkrimson pear.Anthocyanin-rich Starkrimson pears were found to be highly resist-ant to A.niger.Therefore,the anthocyanins of Starkrimson pear were extracted,and Escherichia co-li(E.coli)and A.niger were treated with 0,5%and 10%anthocyanins,respectively,and it was found that 5%and 10%anthocyanins showed different inhibitory effects on both E.coli and A.ni-ger.The Starkrimson pears were infected by A.niger,and the expression changes of anthocyanin-re-lated genes in the infected lesions of pears were analyzed by RT-qPCR.The expression levels of Py-MYB10,PyMYB114,PybHLH3,PyDFR,PyANS and PyUFGT were up-regulated,indicating that pear fruit may inhibit the infection of the postharvest pathogenic fungus A.niger by up-regula-ting the anthocyanin synthesis pathway.

关键词

/花青素/抑菌/黑曲霉/大肠杆菌

Key words

pear/anthocyanidin/bacteriostasis/Aspergillus niger(A.niger)/Escherichia coli(E.coli)

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出版年

2024
合肥工业大学学报(自然科学版)
合肥工业大学

合肥工业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.608
ISSN:1003-5060
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