Preparation Process Optimization and Content Determination of Xuhuang Mixture
To optimize the extraction process of Xuhuang mixture and establish the determination method of content.Taking the content of Astragaloside IV as the index to evaluate the extraction process and content determination,and using the L9(34)orthogonal test to optimize the extraction process with the amount of water,decocting time and decocting times as investigation factors.The effects of concentration at atmospheric and vacuum pressure on the content of Astragaloside IV were investigated.In addition,a method for the detection of Astragaloside IV with high performance liquid chromatography was established and the methodology was investigated.The results showed that the best extraction process was as follow:the herbs were with twice decoction with one hour for each time,and the added water amount was 6-fold of the herbs.Concentration at atmospheric or vacuum pressure had no significant effect on Astragaloside IV’s content in the mixture.Moreover,the linear range was 0.048 16-0.963 20 mg/mL,the negative samples have no interference,and the RSD of precision,repeatability and stability were less than 3%.The average recovery was 101.85%(RSD=2.54%,n=9),which indicated the method was sensitive and accurate.
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