The HPLC method was used to separate and determine the contents of pyrazosulfuron-ethyl,pretilachlor and ouinclorac in the pesticiside granule which contained the active ingredients mentioned above.In the method,the column(50 mm×4.6 mm C18)was used and the mixture of acetonittrile,methanol and 0.05%phosphoric acid solution was used as the mobile phase,and 254nm was the selected wavelength in the determination(the selected wavelength was changed 230 nm 2 min later).The method was validated also and the linear equation of the method for pyrazosulfuron-ethyl is y=3 144x+2.8726,the linear correlation coefficient is R2=0.999 9;the linear equation of the method for pretilachlor is y=426.78x-0.08,the linear correlation coefficient is R2=0.999 9;the linear equation of the method for quinclorac is y=5 630.4x+51.736,the linear correlation coefficient is R2=1.000 0,The validation results show the following characteristics of the method:a good linear relationship,high precision,high recovery and good resolution.The method is suitable for qualitative and quantitative detection.
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