With the strain of recombinant cephalosporium acremonium producing cephalosporin C as the research object,the effect of multiple nitrogen sources on the production of cephalosporin C was studied.Through multi-factor two-level analysis experiment,climbing experiment and response surface method to optimize the fermentation medium,the optimal nitrogen sources were methionine,corn protein powder,and soybean oil.The optimal fermentation medium formula was:0.93 g/L of methionine,4.96 g/L of corn protein powder,and 6.33 g/L of soybean oil.Under this fermentation formula,the yield of cephalosporin C could reach 33.21 g/L,which was 16.24%higher than the original formula.
维普
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