A class of polymeric pH fluorescent probe PRAM was designed and prepared using Rhodamine B and acrylamide as the main raw materials.The structure of PRAM was characterized.The photophysical properties of this probe and the fluorescence imaging of living cells were investigated.The experimental results showed that the p Ka value of the probe was 4.30,and it had high selectivity and sensitivity to H+ in the pH range of 3.0-7.0,allowing real-time monitoring of system pH changes.Furthermore,its fluorescence intensity at 585 nm showed a good linear relationship with pH in the pH range of 5.0-6.0,with a linear correlation coefficient of 0.9955,which could be used to accurately determine the pH of the system.PRAM still performed well in terms of interference resistance and selectivity in the presence of common metal ions.PRAM had good water solubility and membrane permeability,allowing rapid penetration of cell membranes under weakly acidic conditions,and enables fluorescence imaging of pH changes in living cells.Therefore,it could be used to detect physiological pH changes within organelles(e.g.,lysosomes,cancer cells,etc.)in a weakly acidic environment.
关键词
高分子荧光探针/罗丹明B酰肼/pH探针/细胞成像/合成
Key words
polymer fluorescent probe/Rhodamine B hydrazide/pH probes/cell imaging/synthesis
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