Effect of low intensity ultrasound on operation performance of high load Anammox-EGSB reactor
The effect of low intensity ultrasound on the treatment of high-ammonia-nitrogen wastewater by Anammox-EGSB reactor was studied.The effects of ultrasound treatment on the nitrogen removal performance of the reactor,characteristics of Anammox granular sludge,extracellular polymer and microbial flora were investigated.The results showed that low intensity ultrasound could improve the nitrogen removal efficiency of Anammox reactor,and the nitrogen load of influent was 6.03kg N/(m³·d),the total nitrogen removal rate of ultrasonic group was increased by 11.40%,and the impact resistance of nitrogen load was also enhanced.After periodic ultrasonic irradiation,the particle size of granular sludge was maintained at 1.0-1.5mm,which was beneficial to improve mass transfer efficiency,enhance the activity of Anammox granular sludge and reduce particle floating.The total amount of EPS of sludge in ultrasonic group increased significantly,and the increase of TB-EPS was more obvious,which was helpful to maintain the structural stability of granular sludge.The types of functional groups on the sludge surface remained unchanged,but the groups such as hydroxyl,carboxyl,and amino groups increased.The specific Anammox activity of granular sludge increased by 33.2%.Through the simplified Gompertz equation model,it was found that the growth rate of Anammox bacteria in the ultrasonic group(0.0127d-1)was higher than that in the control group(0.0107d-1).High-throughput sequencing showed that ultrasound promoted Anammox bacteria and their symbiotic bacteria,among which Candidatus Brocadia increased by 22.03%.At the same time,some denitrifying bacteria were seriously inhibited,so that the substrate and living space of Anammox bacteria were more sufficient.
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