首页|废水处理硝酸盐异化还原与厌氧氨氧化/反硝化耦合工艺构建

废水处理硝酸盐异化还原与厌氧氨氧化/反硝化耦合工艺构建

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采用厌氧膨胀颗粒污泥床反应器(EGSB)处理含COD、氨氮和硝氮的模拟废水,旨在高浓度有机废水处理系统中快速构建厌氧氨氧化(Anammox)运行工艺.通过接种少量Anammox污泥和逐步提高氨氮浓度的操作方式,在连续运行58d后,系统成功启动Anammox反应,此时的总氮和COD去除率稳定在97%和98%以上.物料衡算显示,Anammox反应途径对氮的去除贡献逐渐增加,硝酸盐异化还原(DNRA)耦合Anammox和反硝化共同促进了系统的同步脱氮除碳.对微生物群落分析发现,Candidatus Kuenenia相对丰度由0.27%快速升高至35.87%,DNRA菌(Ignavibacterium、Thermogutta)及反硝化菌(Azospira、Gp3)在体系内共存.通过基因注释法,检测出了Anammox、DNRA、硝酸盐还原及亚硝酸盐还原关键基因.运行过程中,颗粒污泥颜色变红且粒径增大;胞外聚合物(EPS)分析表明多糖(PS)和蛋白质(PN)含量增加而PN/PS下降;三维荧光光谱发现腐殖酸类物质增多.研究结果为高浓度有机含氮废水高效处理提供了一种新的工艺途径.
Construction of a coupled process integrating dissimilatory nitrate reduction and anaerobic ammonia oxidation/denitrification for wastewater treatment
An anaerobic expanded granular sludge bed reactor(EGSB)was used to treat simulated wastewater containing COD,ammonia and nitrate nitrogen to rapidly establish anaerobic ammonium oxidation(Anammox)process in a high-concentration organic wastewater treatment system.By inoculating a small amount of Anammox sludge and gradually increasing the concentration of ammonia nitrogen,the system successfully started the Anammox reaction after 58d of continuous operation,at which the total nitrogen and COD removal efficiencies were stable to over 97%and 98%,respectively.Mass balance showed that the contribution of Anammox reaction pathway to nitrogen removal gradually increased,and dissimilatory nitrate reduction(DNRA)coupled with Anammox and denitrification together contributed to simultaneous nitrogen and carbon removal in the system.Microbial community analysis found that the relative abundance of Candidatus Kuenenia rapidly increased from 0.27%to 35.87%,and DNRA bacteria(Ignavibacterium,Thermogutta)and denitrification bacteria(Azospira,Gp3)co-existed in the system.The key functional genes of Anammox,DNRA,nitrate reduction and nitrite reduction were detected by gene annotation method.During the operation,the granular sludge turned reddish in color and its particle size increased.Extracellular polymeric substance(EPS)analysis showed an increase in polysaccharide(PS)and protein(PN)contents and a decrease in PN/PS.Three-dimensional fluorescence spectroscopy revealed an increase in humic acid-like substances.These results of the study provide a novel process pathway for efficient treatment of high concentration organic nitrogenous wastewater.

expanded granular sludge bed reactorhigh concentration organic nitrogenous wastewaterdissimilatory nitrate reductionanaerobic ammonia oxidationdenitrificationcoupled nitrogen removal

赵瑞强、周鑫、牛冰心

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太原理工大学环境科学与工程学院,山西晋中 030600

山西省市政工程研究生教育创新中心,山西 晋中 030600

膨胀颗粒污泥床反应器 高浓度有机含氮废水 硝酸盐异化还原 厌氧氨氧化 反硝化 耦合脱氮

国家自然科学基金山西省基础研究计划

2160711120210302123198

2024

10.16085/j.issn.1000-6613.2023-0449

化工进展
中国化工学会,化学工业出版社

化工进展

CSTPCD北大核心
影响因子：1.062
ISSN：1000-6613
年,卷(期)：2024.43(3)
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