Insulin controlled release carriers based on temperature and glucose dual-response copolymer microcapsules
The treatment of diabetes is to seek the carriers that can monitor the blood glucose level immediately and release the required dosage of insulin accurately.Therefore,the development of intelligent insulin controlled release carriers that can self-regulate according to the change of glucose concentration has important clinical application values.This work creates a feasible strategy for the preparation of temperature and glucose dual-response copolymer microcapsules that can be used for insulin controlled release.Firstly,random copolymers with N-isopropylacrylamide(NIPAAm)and 3-acrylamidophenylboronic acid(AAPBA)as the main components were synthesized by free radical polymerization.The surfaces of glass microspheres were covered with copolymer solution by bottom-spray coating technology.Then the copolymers were grafted on the surfaces of the microspheres by heating and annealing,and the templates were dissolved by hydrofluoric acid to form the copolymer microcapsules.The chemical composition,size distribution,equilibrium swelling,surface morphology,stability,cytotoxicity and controlled release capacity of insulin were investigated and analyzed.The results showed that the copolymer microcapsules had relatively uniform size distribution,good stability,no cytotoxicity,significant temperature and glucose sensitivity,and could be applied for loading of insulinoma cells,as well as controlled release of secreted insulin.The scheme for preparing the smart responsive insulin controlled release carriers has access to a powerful tool for creating artificial pancreas.